Genotyping-by-sequencing in Ecological and Conservation Genomics
The fields of ecological and conservation genetics have developed greatly in recent decades through the use of molecular markers to investigate organisms in their natural habitat and to evaluate the effect of anthropogenic disturbances. However, many of these studies have been limited to narrow regions of the genome, allowing for limited inferences but making it difficult to generalize about the organisms and their evolutionary history. Tremendous advances in sequencing technology over the last decade (i.e. next-generation sequencing; NGS) have led to the ability to sample the genome much more densely and to observe the patterns of genetic variation that result from the full range of evolutionary processes acting across the genome. These studies are transforming molecular ecology by making many long-standing questions much more easily accessible in almost any organism.
Narum, S.R., C.A. Buerkle, J.W. Davey, M.R. Miller and P.A. Hohenlohe. 2013. Genotyping-by-sequencing in ecological and conservation genomics. Molecular Ecology 22:2841–2847.