Transcriptomic Response to Heat Stress Among Ecologically Divergent Populations of Redband Trout
As ectothermic organisms have evolved to differing aquatic climates, the molecular basis of thermal adaptation is a key area of research. In this study, we tested for differential transcriptional response of ecologically divergent populations of redband trout (Oncorhynchus mykiss gairdneri) that have evolved in desert and montane climates. Each pure strain and their F1 cross were reared in a common garden environment and exposed over four weeks to diel water temperatures that were similar to those experienced in desert climates within the species’ range. Gill tissues were collected from the three strains of fish (desert, montane, F1 crosses) at the peak of heat stress and tested for mRNA expression differences across the transcriptome with RNA-seq.
Narum, S.R. and N.R. Campbell. 2015. Transcriptomic response to heat stress among ecologically divergent populations of redband trout. BMC Genomics 2015 16:103.